Jem Biotech KTS-01說明書

更新時間：2018-10-27

點擊次數：2005

Jem Biotech Inc.是一家綜合性學科，提供生物技術和化學領域的廣泛服務。我們的主要目標是成為您的研究合作伙伴。我們是印度Microtest Innovations的主要合作伙伴。

Jem Biotech KTS-01說明書

KlenTaq-S酶——適PAP聚合酶

PAP(焦磷酸化激活的聚合反應法)是一種*的DNA擴增技術（Liu and Sommer: BioTechniques, 29 [2000], 1072-1080; Nucleic Acids Res, 30 [2002], 598-604),該技術利用DNA聚合酶在3'封閉的引物進行連續的焦磷酸化和聚合反應。焦磷酸化是DNA聚合的逆反應，其引物模板雙鏈的3'末端封閉核苷酸（通常是雙脫氧）在無機焦磷酸鹽存在下被酶解清除，在焦磷酸化清除封閉的核苷酸后，激活引物進行后續的DNA聚合。由于PAP的高特異性（1 / 3*10¹¹），該技術正成為檢測存在大量野生型等位基因的情況下檢測稀有突變，大雜合缺失和重復基因的有力工具（Liu et al：Biotechniques， 40 [2006]，661-668）。理論上，PAP可以檢測3*10¹¹個拷貝的野生型等位基因中的單堿基突變，此外，PAP還可用作替代或證實real-time PCR反應 (Boon et al : Prenat Diagn 27[2007], 932-937)。

KlenTaq-S酶：

KlenTaq-S已被多個研究團隊證實是PAP反應適的熱穩定Taq DNA聚合酶，該酶由5'缺失和F667Y（Tabor和Richardson）突變的基因表達，編碼的Thermus aquaticus DNA聚合酶。該酶反復暴露于98度的PR1反應緩沖液中沒有表現出任何明顯的活性損失，具有*的熱穩定性。

KlenTaq-S酶的濃度 : 10 U/µl

建議PAP反應條件 (25 µl反應體系) :

50 mM Tris-HCl, 7.8,

16 mM 硫酸銨,

3.5 mM 氯化鎂,

25 µM each four dNTPs,

0.1 µM 引物,

5-200 ng模板,

90 µM 無機焦磷酸鹽 (Na4PPi),

2% DMSO,

循環條件 :

95度1 min (變性),

94度 15 sec

60度 30 sec

64度 30 sec

68度 1 min

72度 1-2 min

循環數25-45 。

緩沖體系 (10X PR1):

500 mM Tris-HCl, pH 7.8,

160 mM (NH4)2SO4,

35 mM MgCl2, 1.5 mg/ml BSA

訂貨信息 :

貨號 : KTS-01

含量: 100 µl or 1000 U/vial

價格 : $200/vial (1000 U)

jembio主要產品：

Item Code	Products	Host	Amount	Price ($)
LA001	Acid Phosphatase, Polyclonal anti-Human Affinity Purified	Host Chicken	5 mg	1300
LA002	Albumin, Polyclonal anti-Human Affinity Purified (ALB)	Host Goat	10 mg	800
LA003	Albumin, Polyclonal anti-Human Affinity Purified (ALB)	Host Chicken	10 mg	600
LA004	Alpha Amylase, Polyclonal anti-Human (whole antiserum)	Host Rabbit	50 ml	900
LA005	Alpha Fetoprotein, Polyclonal anti-Human (AFP) Whole Antiserum	Host Goat	50 ml	900
LA006	Alpha-1 Antitrypsin, Polyclonal anti-Human Affinity Purified (A1AT)	Host Goat	10 mg	700
LA007	Alpha-1 Antitrypsin, Polyclonal anti-Human Affinity Purified (A1AT)	Host Chicken	10 mg	700
LA008	Alpha-1-Acid Glycoprotein, anti-Human Affinity Purified (A1AG)	Host Goat	10 mg	750
LA009	alpha-2-Macroglobulin, Polyclonal anti-Human affinity Purified (A2M)	Host Chicken	10 mg	700
LA010	anti-Feline Leukemia Virus p27, Monoclonal	Tissue Culture	5 mg	3000
LA011	anti-Feline Leukemia Virus p27, Monoclonal-CMI	Tissue Culture	5mg	3000
LA012	beta 2-Microglobulin ,anti-Human HRP Conjugated	Host Chicken	10 mg	1400
LA013	beta 2-Microglobulin, Polyclonal anti-Human Affinity Purified (B2M)	Host Chicken	10 mg	650
LA014	BNP Polyclonal anti-human affinity purified	Host Rabbit	5 mg	3000
LA015	C-Reactive Protein (CRP), Polyclonal anti-Rat affinity purified	Host Rabbit	1 mg	1500
LA016	C-Reactive Protein, Polyclonal anti-Human affinity purified (CRP)	Host Goat	10 mg	750
LA017	C3, Polyclonal anti-Human Affinity Purified	Host Chicken	10 mg	750
LA018	C4 , Polyclonal anti-Human Affinity Purified	Host Goat	10 mg	750
LA019	Cathepsin, Polyclonal anti-Human Affinity Purified	Host Chicken	5 mg	1450
LA020	Ceruloplasmin, Polyclonal anti-Human Affinity Purified	Host Chicken	10 mg	750
LA021	Elastase, anti-Human IgG Fraction	Host Sheep	100 mg	500
LA021	Elastase, anti-Human IgG Fraction	Host Sheep	100 mg	500
LA022	Elastase, Polyclonal anti-human (whole anti-serum)	Host Sheep	30 ml	400
LA023	Ferritin, Polyclonal anti-Human Affinity Purified	Host Rabbit	10mg	1750
LA024	Goat anti-Human Whole Serum	Host Goat	100 ml	1750
LA025	Haptoglobin Polyclonal anti-human (whole antiserum)	Host Goat	10 ml	500
LA026	Haptoglobin, Polyclonal anti-Human Affinity Purified (Hp)	Host Goat	10 mg	1200
LA027	Haptoglobin, Polyclonal anti-Human Affinity Purified (Hp)	Host Rabbit	10 mg	1200
LA028	Heartworm,.Polyclonal anti-Canine Affinity Purified	Host Chicken	10 mg	3000
LA029	Heartworm,Polyclonal anti-Canine,HRP Conjugated	Host Chicken	10 mg	3500
LA030	Hemoglobin, Polyclonal anti-Human Affinity Purified	Host Goat	30 ml	750
LA031	Hemoglobin, Polyclonal anti-Human Affinity Purified	Host Sheep	10 mg	750
LA032	IgA, Polyclonal anti-Human Affinity Purified	Host Chicken	10 mg	750
LA033	Kappa, Polyclonal anti-Human Affinity Purified	Host Chicken	10 mg	1000
LA034	Kappa, Polyclonal anti-Human Affinity Purified	Host Goat	10 mg	750
LA035	Lactate Dehydrogenase , Polyclonal anti-Human Affinity Purified (LDH)	Host Chicken	10 mg	3500
LA036	Lactoferrin Polyclonal anti-Human Affinity Purified	Host Goat	10 mg	1300
LA037	Lactoferrin, Polyclonal Whole anti-serum	Host Rabbit	50 ml	1000
LA038	Lambda, Polyclonal anti-Human Affinity Purified	Host Chicken	10 mg	1000
LA039	Lambda, Polyclonal anti-Human Affinity Purified	Host Goat	10 mg	750
LA040	Myeloperoxidase, Polyclonal anti-human affinity purified	Host Chicken	10 mg	3500
LA041	Myeloperoxidase, Polyclonal anti-Human affinity purified (MPO)	Host Rabbit	10 mg	3500
LA042	Myoglobin Polyclonal anti-human affinity purified	Host Chicken	10 mg	2500
LA043	Myoglobin, Polyclonal anti-Human Affinity Purified	Sheep	10 mg	5000
LA044	Prostate Specific Antigen,(PSA) Polyclonal anti-Human Affinity Purified	Host Chicken	5 mg	2300
LA045	Rabbit anti-Human Whole Serum	Host Rabbit	50 ml	2000
LA046	Retinol Binding Protein ,Polyclonal anti-Human Affinity Purified (RBP)	Host Chicken	10 mg	3200
LA047	Transferrin, Polyclonal anti-Human Affinity Purified	Host Chicken	10 mg	750
LA048	Troponin I, anti-human whole antiserum	Host Rabbit	500 ml	15000
MMT001	α His tag, monoclonal		Inquire	Inquire
MMT002	α Taq Pol, monoclonal		Inquire	Inquire
MMT003	α KlenTaq Pol, monoclonal		Inquire	Inquire
MMT004	α HIV P24, monoclonal		Inquire	Inquire
MMT005	α CD3, monoclonal		Inquire	Inquire
MMT006	α CD4, monoclonal		Inquire	Inquire

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