VisualProtein BC03-500說(shuō)明書

更新時(shí)間：2019-06-26

點(diǎn)擊次數(shù)：1746

Visual Protein成立于2005年，致力于通過(guò)為研究提供創(chuàng)新產(chǎn)品和服務(wù)來(lái)加強(qiáng)生物開發(fā)。我們的優(yōu)勢(shì)是我們的研發(fā)能力。Visual Protein開發(fā)的大多數(shù)產(chǎn)品比公司開發(fā)的產(chǎn)品更有效。我們的專業(yè)知識(shí)拓寬了蛋白質(zhì)組學(xué)，免疫學(xué)和細(xì)胞學(xué)領(lǐng)域的研究可能性。

VisualProtein BC03-500說(shuō)明書

Dual-Range ™ BCA蛋白質(zhì)分析試劑盒

常用的測(cè)量蛋白質(zhì)濃度的方法

Dual-Range™BCA蛋白質(zhì)分析試劑盒是常用的蛋白質(zhì)分析方法。它基于二辛可寧酸（BCA），用于比色檢測(cè)和定量5-2,000μg/ mL濃度范圍內(nèi)的總蛋白。與Lowry方法一樣，該分析依賴于蛋白質(zhì)對(duì)Cu ²⁺離子的還原。由此形成的Cu ⁺通過(guò)與二辛可寧酸反應(yīng)轉(zhuǎn)化成紫色物質(zhì)來(lái)檢測(cè)。Dual-Range™BCA蛋白質(zhì)分析試劑盒與許多洗滌劑兼容，但與DTT，DTE和2-巰基乙醇等還原劑不相容。

BCA蛋白質(zhì)測(cè)定基于堿性溶液中蛋白質(zhì)將Cu 2+還原為Cu +，通過(guò)二辛可寧酸（BCA）對(duì)Cu +進(jìn)行靈敏且特異性的比色檢測(cè)。兩個(gè)BCA分子與每個(gè)Cu +螯合，使還原的銅從蘋果綠色變?yōu)樽仙珡?fù)合物，在562nm處具有強(qiáng)吸收。

特色

寬線性范圍：標(biāo)準(zhǔn)方案為20-2000μg/ mL，增強(qiáng)方案為5-250μg/ mL
兼容：不受大多數(shù)??離子和非離子洗滌劑的典型濃度影響

訂單信息

貨號(hào)

產(chǎn)品名稱

描述

BC03-500

Dual-Range™BCA蛋白質(zhì)分析試劑盒

1個(gè)套件

500mL試劑A + 12 mL試劑

B + 10mL BSA（2mg / mL）

用于制作2500個(gè)微孔板測(cè)定

產(chǎn)品明細(xì)

圖1.通過(guò)使用微孔板程序（37℃，30分鐘）的牛血清白蛋白（BSA）和牛γ球蛋白（BGG）的標(biāo)準(zhǔn)曲線。

圖2.通過(guò)使用微孔板程序（37℃，30分鐘），用5％Triton X-100或8％SDS的BSA的標(biāo)準(zhǔn)曲線。

圖3.通過(guò)使用
具有增強(qiáng)方案的微孔板程序（60℃持續(xù)30分鐘）使用微孔板程序的BSA的標(biāo)準(zhǔn)曲線。

貨號(hào)	品名	規(guī)格
BC03-500	Dual-RangeTM BCA protein assay kit	1 kit
BR05-500	Bradford Reagent (5X)	1 kit
BR05-500-K	Bradford Protein Assay Kit (5X)	1 kit
BP01-1L	BlockPROTM Blocking Buffer	1 kit
BF01-1L	BlockPROTM Protein-Free Blocking Buffer	1 kit
BF10-100	BlockPROTM Protein-Free Blocking Buffer (10X)	1 kit
BF20-50P	BlockPROTM Protein-Free Blocking Buffer (20X)	1 kit
BM01-500	BlockPRO 1 Min Protein-Free Blocking Buffer	1 kit
BM10-100	BlockPRO 1 Min Protein-Free Blocking Buffer (10X)	1 kit
BOX12	Western Blot Tool Box	1 kit
BOX12-03	Western Blot Tool Box	1 kit
EP05-30	ExtractPROTM Protein Extraction Reagent (Phenol included)	1 bottle
FP01-5	FracPROTM Protein Fractionation Kit	1 kit
FP01-50	FracPROTM Protein Fractionation Kit	1 kit
HB01-1L	HybriMoreTM Hybridoma Cloning Factor	1 bottle
IF01-4N	ImmunoFastTM Adjuvant	1 kit
IF01-20N	ImmunoFastTM Adjuvant	1 kit
LF01-10S	LumiFlashTM Prime Chemiluminescent Substrate, HRP (sample size)	1 kit
LF01-100	LumiFlashTM Prime Chemiluminescent Substrate, HRP	1 kit
LF01-500	LumiFlashTM Prime Chemiluminescent Substrate, HRP	1 kit
LF08-10S	LumiFlashTM Ultima Chemiluminescent Substrate, HRP (sample size)	1 kit
LF08-100	LumiFlashTM Ultima Chemiluminescent Substrate,HRP	1 kit
LF08-500	LumiFlashTM Ultima Chemiluminescent Substrate,HRP	1 kit
LF16-10S	LumiFlashTM Infinity Chemiluminescent Substrate,HRP (sample size)	1 kit
LF16-100	LumiFlashTM Infinity Chemiluminescent Substrate, HRP	1 kit
LF16-500	LumiFlashTM Infinity Chemiluminescent Substrate, HRP	1 kit
LH03-10	LuminolPenTM, HRP System	1 pen
LH03-50	LuminolPenTM, HRP System	1 pen
PBS10-1L	PBS Buffer, 10X (pH 7.4)	1 kit
PBST10-1L	PBS Buffer, 10X (pH 7.4), with 0.5% Tween 20	1 kit
PP03-2C	PhosPROTM Phosphoprotein Enrichment Kit	1 kit
PP03-5E	PhosPROTM phosphoprotein Enrichment Kit	1 kit
PP03-6C	PhosPROTM Phosphoprotein Enrichment Kit	1 kit
PP05-2C	PhosPROTM Phosphoprotein Purification Kit	1 kit
PP05-5E	PhosPROTM Phosphoprotein Purification Kit	1 kit
PP05-6C	PhosPROTM Phosphoprotein Purification Kit	1 kit
RB10-1L	SDS-PAGE Running Buffer (Tris-Glycine, pH 8.3), 10X	1 kit
RB500P	SDS-PAGE Running Buffer (Tris-Glycine), powder pack	1 kit
RP05-10	5X RIPA Cell Lysis Buffer (sample size)	1 bottle
RP05-100	5X RIPA Cell Lysis Buffer	1 bottle
RP05-500	5X RIPA Cell Lysis Buffer	1 bottle
SBN06-15	6x Laemmli SDS Sample Buffer, non-reducing	1 kit
SBR06-15	6x Laemmli SDS Sample Buffer, reducing	1 kit
SP05-100	StripPRO 1 Min Stripping Buffer (5X)	1 kit
TBS10-1L	TBS Buffer, 10X (pH 7.4)	1 kit
TBST10-1L	TBS Buffer, 10X (pH 7.4), with 0.5% Tween 20	1 kit
TBST200P	TBS Buffer, 25X (pH7.4), with 0.5% Tween 20	1 kit
VC01-25S	VisColorTM Pre-Stained Protein Marker	1 vial
VC01-250	VisColorTM Pre-Stained Protein Marker	1 vial
VC01-500	VisColorTM Pre-Stained Protein Marker	2 vials
VC03-25S	VisColorTM Full-Range Pre-Stained Protein Marker	1 vial
VC03-250	VisColorTM Full-Range Pre-Stained Protein Marker	1 vial
VC03-500	VisColorTM Full-Range Pre-Stained Protein Marker	2 vials
VP01-125	VisPROTM 5 Minutes Protein Stain Kit (sample size)	1 kit
VP01-500	VisPROTM 5 Minutes Protein Stain Kit	1 kit
VP05-125	5X VisPROTM 5 Minutes Protein Stain Kit (sample size)	1 kit
VP05-500	5X VisPROTM 5 Minutes Protein Stain Kit	1 kit
WTB10-1L	Western Blot Transfer Buffer (Tris-Glycine, pH 8.3), 10X	1 kit
WTB500P	Western Blot Transfer Buffer, powder pack	1 kit
BSB01	Black Staining Box (for VisPRO Protein Stain Kit)	1 box
BSB02	Black Staining Box (for VisPRO Protein Stain Kit)	1 box
BSB03	Black Staining Box (for VisPRO Protein Stain Kit)	1 box
BSB04	Black Staining Box (for VisPRO Protein Stain Kit)	1box

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